Non-Esterified Fatty Acid (NEFA)

FF_Wako_IVD_NEFA_HR2 Product Photo

HR Series NEFA-HR(2)

For In Vitro Diagnostic Use


Intended Use

The Wako HR series NEFA-HR(2) is an in vitro enzymatic colorimetric method assay for the quantitative determination of non-esterified fatty acids (NEFA) in serum.



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Special Features


  • Detects a variety of free fatty acids
  • Broad Linear Range (0.01- 4.00 mEq/L)
  • No extraction step required
  • Designed for automated analyzers

Measurements of fatty acids are used in the diagnosis and treatment of various disorders of lipid metabolism. Labor intensive extraction/ titration methods have been commonly applied to the measurement of free fatty acids in serum. These approaches are time consuming, hazardous and not easily automated. The FUJIFILM Wako’s NEFA assay is an original test developed as an enzymatic method which is available in a series of individual reagents. The need for an extraction step has been eliminated. This enzymatic method is accurate, precise, simple and fast. The FUJIFILM Wako’s method relies upon the acylation of coenzyme A (CoA) and ultimately results in a purple colored product which can be measured colorimetrically at 550 nm.



Automated Analyzer Applications

To request an automated analyzer application, please email us at: Customer Service.
Please include the names of the product and the analyzer/instrument.

NEFA HR(2) Family Product Information

Catalog No. Product Name Pkg Size Storage Condition
999-34691 HR Series NEFA-HR(2) Color Reagent A 4 x for 50 mL 2-10°C
995-34791 HR Series NEFA-HR(2) Solvent A 4 x 50 mL 2-10°C
991-34891 HR Series NEFA-HR(2) Color Reagent B 4 x 25 mL 2-10°C
993-35191 HR Series NEFA-HR(2) Solvent B 4 x 25 mL 2-10°C
276-76491 NEFA Standard Solution
Related Products
997-76491 Wako NEFA Linearity Set