Free Cholesterol

    Free Cholesterol E

    For Research Use Only. Not for use in diagnostic procedures.

    Intended Use

    The Wako Free Cholesterol E is an in vitro assay for the quantitative determination of free cholesterol in serum.


    Enzymatic Colorimetric Method

    Special Features

    • Complete kit with reagents and standard
    • Manual and automated measurement of Cholesterol in serum
    • Linear up to 300 mg/dL

    Cholesterol concentration in serum is closely related to the formation, absorption and catabolism of cholesterol in liver and in intestine and to the metabolism of lipoproteins in blood.  

    Early methods for determining cholesterol levels were based on chemical colorimetric methods. FUJIFILM Wako developed a Free Cholesterol test kit that is based on an enzymatic method, employing 3,5-dimethoxy-N-ethyl-N-(2-hydroxy-3-sulfopropyl) aniline sodium (DAOS) that produces blue pigment. The FUJIFILM Wako Free Cholesterol E assay is an in vitro assay for the quantitative determination of free cholesterol in serum.


    Automated Analyzer Applications

    To request an automated analyzer application, please email us at: Customer Service.

    Please include the names of the product and the analyzer/instrument.

    Microplate Application

    Free Cholesterol E Microtiter Procedure

    Product Information

    Catalog No. Product Name Pkg Size Storage Condition
    993-02501 Free Cholesterol E Buffer Solution:
    2 x 75 mL
    Color Reagent:
    2 x for 75 mL
    Standard Solution:
    1 x 10 mL
    Calibrator is included in the kit.


    Package Insert

    Free Cholesterol E

    Product Brochure

    Free Cholesterol

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